Mushrooms Lesson 8 — Fungi Kingdom:
Technique[ edit ] Streaking is rapid and ideally a simple process of isolation dilution. The technique is done by diluting a comparatively large concentration of bacteria to a smaller concentration. The decrease of bacteria should show that colonies are sufficiently spread apart to effect the separation of the different types of microbes.
Streaking is done using a sterile tool, such as a cotton swab or commonly an inoculation loop.
Aseptic techniques are used to maintain microbiological cultures and to prevent contamination of the growth medium.
There are many different types of methods used to streak a plate.
Picking a technique is a matter of individual preference and can also depend on how large the number of microbes the sample contains.
Illustration of streak plate procedure to achieve isolated colonies using aseptic technique. The three-phase streaking pattern, known as the T-Streak, is recommended for beginners.
The streaking is done using a sterile tool, such as a cotton swab or commonly an inoculation loop. The inoculation loop is first sterilized by passing it through a flame. Lab 1 the scientific method the loop is cool, it is dipped into an inoculum such as a broth or patient specimen containing many species of bacteria.
The loop then is re-sterilized and the plate is turned 90 degrees. Starting in the previously streaked section, the loop is dragged through it two to three times continuing the zigzag pattern.
The procedure is then repeated once more being cautious to not touch the previously streaked sectors. Each time the loop gathers fewer and fewer bacteria until it gathers just single bacterial cells that can grow into a colony. The plate should show the heaviest growth in the first section.
The second section will have less growth and a few isolated colonies, while the final section will have the least amount of growth and many isolated colonies. Growth medium[ edit ] The sample is spread across one quadrant of a petri dish containing a growth medium.
Bacteria need different nutrients to grow. This includes water, a source of energy, sources of carbon, sulfur, nitrogen, phosphorus, certain minerals, and other vitamins and growth factors.
A very common type of media used in microbiology labs is known as agara gelatinous substance derived from seaweed. The nutrient agar has a lot of ingredients with unknown amounts of nutrients in them. On one hand, this can be a very selective media to use because as mentioned bacteria are particular.
If there is a certain nutrient in the media the bacteria could most certainly not grow and could die. On the other hand, this media is very complex. Complex media is important because it allows for a wide range of microbial growth. The bacteria growth can be supported by this media greatly due in part to the high amounts of nutrients.
Choice of which growth medium is used depends on which microorganism is being cultured, or selected for.
Different labs have different standards as to the direction and style of the streaking. Incubation[ edit ] Dependent on the strain, the plate may then be incubatedusually for 24 to 36 hours, to allow the bacteria to reproduce.
At the end of incubation there should be enough bacteria to form visible colonies in the areas touched by the inoculation loop. Automated equipment is used at industrial level for streak plating the solid media in order to achieve better sterilization and consistency of streaking and for reliably faster work.
While streaking manually it is important to avoid scratching the solid medium as subsequent streak lines will be damaged and non-uniform deposition of inoculum at damaged sites on the medium yield clustered growth of microbes which may extend into nearby streak lines.
Importance[ edit ] Bacteria exist in water, soil and food, on skin, and intestinal tract normal flora. The assortment of microbes that exist in the environment and on human bodies is enormous.
The human body has billions of bacteria which creates the normal flora fighting against the invading pathogens. Bacteria frequently occur in mixed populations.An investigation is a project where the problem, or answer to a question, is solved.
When undertaking an investigation, a method is followed that allows for the testing of an idea, or finding a solution to a problem, which determines a clear conclusion to the problem or question asked. Choose the correct answer for each question.
Show all questions ? spatula? beaker tongs? crucible tongs.
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